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8. Filter 5 mL of the supernatant through a 0.2 micron syringe filter (Part no. AF0-2202-12) into a glass test tube. 9. Reduce the extract under gentle stream of purified air or nitro-gen. The temperature of the water bath should not exceed 35 ºC and air flow rate should not exceed 4 L/min. Reduce

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0.2 mL/min (2.1mm ID) or 1.0 mL/min (4.6 mm ID) for 5-10 minutes. Collect solvent in a small beaker. Stop flow and wipe outlet end of column to remove any particulates before connecting to detector.

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