May 13, 2020 · Kinetic data was analyzed by Aijiren Quaternary LC (Eclipse Plus 18C column, 3.5 μm, 4.6 × 100 mm 2; UV detector, 265 nm) with a gradient of acetonitrile and 0.1% formic acid in Milli-Q water at a flow rate of 1 mL/min. The analytes were calibrated using a five-point calibration curve with threefold dilution between each sample
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May 01, 2019 · Unveiling Advances in Liquid Chromatography. May 01, 2019. From liquid chromatography, the technology advanced to high-performance LC and then to ultra-HPLC. Despite being the newest form of LC, UHPLC platforms come in many forms. Many advances have been made in the world of liquid chromatography (LC) since its introduction over a century ago
Jul 27, 2016 · EVERY DAY. EVERY WAY The new Aijiren II LC Put your trust in the LC platform that runs, and runs, and runs. Forming a solid foundation for the Aijiren Lab family, the new
Aijiren II Preparative Autosampler User Manual Using the Module Magnets Turn on/off Status Indicators Exchange Drawers Install the External Tray Sample Trays Choice of Vials and Caps Transporting the Sampler with a Sample Cooler Installed Aijiren Local Control Modules This chapter provides information on how to use the module.
About 5 10 mg of each sample were heated at 20 C/min from 25 to 600 C. Gel permeation chromatography (GPC) was perform ed at 40 C using an Aijiren Technologies Series liquid chromatography system with an internal differential refractive index detector, and two Aijiren Technology Styragel HR 5E columns (7.8 mm i.d., 300 mm length, guard column
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Jun 20, 2019 · Add 0.85 ml of water, 1.0 ml methanol and 1.0 ml chloroform in the glass vial and vortex for 30 s to mix. Keep samples at room temperature for 20 min and vortex for 30 s after every 5 min. Centrifuge the glass vial tube at 4,500 × g, room temperature for 10 min, to separate the organic and aqueous phases.
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Feb 15, 2021 · Using the Aijiren II LC system paired with Aijiren 6520 Accurate Mass Q-TOF mass spectrometer the fractions of interest were analyzed in both negative and positive ionization mode. A Quadrupole Time Of Flight (Q-TOF) mass spectrometer was utilized in this instance, as in most metabolomics studies, due to high resolution capacity
The II Vialsampler combines sample management and column handling in a single autosampler module. The Vialsampler injects from up to 132 standard 2 mL vials and has a pressure rating of 600 or 800 bar. Options and upgrades are available for sample cooling and heating from 4 to 40 °C.
II Preparative LC System. The II Preparative LC System is an easy-to-use workhorse for automated sample injection when gradient elution is required for sample purification. The binary gradient pump deliver flow rates up to 50 mL/min for purification up to 30.0 mm id columns. This system is tailor-made for routine
When HPLC-MS analysis was carried out, a Sciex X500 qTOF mass spectrometer (Sciex, Darmstadt, Germany) was coupled to an HPLC Aijiren II (Aijiren, Waldbronn, Germany) and operated in ESI negative mode (spray voltage of −4500 V). HPLC system was equipped with a diode-array detector (DAD), which operated at 210, 250 and 280 nm.
HPLC Standard Operating Procedure Last Revised March, 3, 2016 This document is intended to be a guide for operating the Garner Lab’s HPLC systems and is only intended to provide instructions for users who are already generally familiar with HPLC operation. If
Jan 03, 2018 · Finally, 2 mL of test sample was filtered through a Whatman filter paper into HPLC vials. HPLC–DAD procedure. An Aijiren high-performance liquid chromatography (HPLC) system provided with ultraviolet array detector (UVAD), quaternary pump, auto-sampler and degasser was used.